Bacterial 16S rDNA sequences in immature volcanic ash soil on volcanoes Mt. Sakurajima and Mt. Fugen in Japan determined by PCR amplification
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چکیده
منابع مشابه
Simultaneous detection of microorganisms in soil suspension based on PCR amplification of bacterial 16S rRNA fragments.
The effect of buffer composition on simultaneous PCR amplification of 16S rRNA gene fragments of five bacterial species was examined using a number of different buffer systems. Tris-based PCR buffers at final concentrations of 10 mM proved unreliable. However, when the final concentration of Tris was increased to 75 mM, all five samples were routinely detected. The use of other buffers, 3-[(1,1...
متن کاملComparison of 16S rDNA-PCR Amplification and Culture of Cerebrospinal Fluid for Diagnosis of Bacterial Meningitis
OBJECTIVE Early and accurate diagnosis of bacterial meningitis is of critical concern. Optimum and rapid laboratory facilities are not routinely available for detecting the etiologic agents of meningitis. The objective of this study was to compare polymerase chain reaction (PCR) assay with culture for detection of bacteria in central nervous system (CNS) samples from patients suspected to have ...
متن کاملBacterial diversity in a soil sample from a subtropical Australian environment as determined by 16S rDNA analysis.
In order to investigate the genetic diversity of streptomycetes in an acid forested soil sample from Mt. Coot-tha, Brisbane, Australia, cells were mechanically lysed within the soil matrix and genomic DNA was isolated and purified. 16S ribosomal (r)DNA was amplified by the polymerase chain reaction (PCR) method using one primer conserved for members of the domain Bacteria and a second designed ...
متن کاملMolecular Diagnosis of Helicobacter Pylori Strain by 16S rDNA PCR Amplification and Direct Sequencing
AIM Rapid detection of H.pylori strains by PCR-Sequencing. METHODS 16S rDNA amplification by PCR from template genomic DNA, confirmation of amplicon size by agarose gel electrophoresis, sequencing of amplicons by automated sequencer, analysis of sequences by NCBI -BLAST software. RESULTS The PCR -Sequencing and analysis of the sequence data by BLAST resulted in detection of the strain to be...
متن کاملIncreased Mortality of Respiratory Diseases, Including Lung Cancer, in the Area with Large Amount of Ashfall from Mount Sakurajima Volcano
OBJECTIVES Mount Sakurajima in Japan is one of the most active volcanoes in the world. This work was conducted to examine the effect of volcanic ash on the chronic respiratory disease mortality in the vicinity of Mt. Sakurajima. METHODS The present work examined the standardized mortality ratios (SMRs) of respiratory diseases during the period 1968-2002 in Sakurajima town and Tarumizu city, w...
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ژورنال
عنوان ژورنال: Soil Science and Plant Nutrition
سال: 1998
ISSN: 0038-0768,1747-0765
DOI: 10.1080/00380768.1998.10414498